Recovery of Ascorbic Oxidoreductase from Crude Extract with an Aqueous Two-phase System in a Perforated Rotating Disc Contactor

نویسندگان

  • Ana Lúcia Figueiredo Porto
  • Leonie Asfora Sarubbo
  • Keila Aparecida Moreira
  • Homero José Farias de Melo
  • José Luís Lima-Filho
  • Galba Maria Campos-Takaki
  • Elias Basile Tambourgi
چکیده

A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The best recovery of enzyme activity was 236%, with a purification factor of 34 in flow rates of 1 mL/min for dispersed phase.

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تاریخ انتشار 2004